Difference between revisions of "Part:BBa M50050:Design"
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=== Special Notes=== | === Special Notes=== | ||
| − | Designing this part required no modifications. During our design process in DNA 2.0, we ran the sequence through codon optimization and tested the restriction sites, but there were no recommendations that required action. | + | Designing this part required no modifications. During our design process in DNA 2.0, we ran the sequence through codon optimization and tested the restriction sites, but there were no recommendations that required action. Our inspiration for this project stemmed from reading the literature about possible sources for possible endogenous pathways for the control of bacterial growth. Some papers described quorum sensing pathways, specifically Autoinducer-2, as having metabolic and growth control properties (See reference 1). |
| − | + | ||
===Plasmid Map=== | ===Plasmid Map=== | ||
| − | [[File:BBa M50050 Plasmid Map.png| | + | [[File:BBa M50050 Plasmid Map.png|500px]] |
===Source=== | ===Source=== | ||
| − | The source of the LuxS gene came from UniProt. All other parts were provided through DNA 2.0 under the plasmid name PD441-CH. | + | The source of the LuxS gene came from UniProt under the name P45578. All other parts were provided through DNA 2.0 under the plasmid name PD441-CH. |
===References=== | ===References=== | ||
| + | 1. Xavier KB and Bassler BL. 2005. Regulation of Uptake and Processing of the Quorum-Sensing Autoinducer AI-2 in Escherichia coli. J Bacteriol. 187(1): 238-248. | ||
Latest revision as of 07:47, 12 December 2016
IPTG-Inducible LuxS Expression in E. coli for Controlling Growth Rates
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 696
Illegal PstI site found at 202 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 696
Illegal PstI site found at 202 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 307
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 696
Illegal PstI site found at 202 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 696
Illegal PstI site found at 202
Illegal AgeI site found at 405 - 1000COMPATIBLE WITH RFC[1000]
Design
Special Notes
Designing this part required no modifications. During our design process in DNA 2.0, we ran the sequence through codon optimization and tested the restriction sites, but there were no recommendations that required action. Our inspiration for this project stemmed from reading the literature about possible sources for possible endogenous pathways for the control of bacterial growth. Some papers described quorum sensing pathways, specifically Autoinducer-2, as having metabolic and growth control properties (See reference 1).
Plasmid Map
Source
The source of the LuxS gene came from UniProt under the name P45578. All other parts were provided through DNA 2.0 under the plasmid name PD441-CH.
References
1. Xavier KB and Bassler BL. 2005. Regulation of Uptake and Processing of the Quorum-Sensing Autoinducer AI-2 in Escherichia coli. J Bacteriol. 187(1): 238-248.