Difference between revisions of "Part:BBa M50021"
 
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<partinfo>BBa_M50021 short</partinfo>
 
<partinfo>BBa_M50021 short</partinfo>
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Combines heat-sensitive sigma32-induced promoter with strong RBS and d-limonene synthase to allow for heat-sensitive limonene production
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Combines heat-sensitive sigma32-induced promoter with strong RBS and d-limonene synthase followed by transcriptional terminator to allow for heat-sensitive limonene catalysis.  Composed (in order) of BBa_50017, BBa_50018, BBa_50019, and BBa_50049
  
  

Latest revision as of 19:09, 11 December 2016


Heat-inducible d-Limonene synthase

Combines heat-sensitive sigma32-induced promoter with strong RBS and d-limonene synthase followed by transcriptional terminator to allow for heat-sensitive limonene catalysis. Composed (in order) of BBa_50017, BBa_50018, BBa_50019, and BBa_50049


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1895
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1600
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1597
    Illegal SapI.rc site found at 319
    Illegal SapI.rc site found at 1572