Difference between revisions of "Part:BBa K1955003"
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<b style="font-size:23px;">pSB1C3-5'HYG</b><br><br> | <b style="font-size:23px;">pSB1C3-5'HYG</b><br><br> | ||
− | We | + | We replaced p36 and nagt genes with a cloning site for an exogenous gene and with hyg as a selection marker, respectively. This dual-function biobrick enables stable expression of foreign proteins by drug selection in Leishmania.<br><br> |
− | + | ||
<b style="font-size:20px;">(1) The basic part checked by PCR: </b> | <b style="font-size:20px;">(1) The basic part checked by PCR: </b> |
Latest revision as of 08:12, 5 December 2016
pSB1C3-5'HYG
We replaced p36 and nagt genes with a cloning site for an exogenous gene and with hyg as a selection marker, respectively. This dual-function biobrick enables stable expression of foreign proteins by drug selection in Leishmania.
(1) The basic part checked by PCR:
We used pSB1C3-5’HYG, pSB1C3-3’UTR, pSB1C3-HA, pSB1C3-OVA as template, to check the length of the inserts. The PCR reaction was performed with Taq polymerase, and screened in 0.8% agarose gel by electrophoresis.
Sequence and Features