Difference between revisions of "Part:BBa K2012020"
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This generator is altered from BBa_K819010 created by Peking University.Its previous promotor , J23113,is replaced by a slightly stronger promotor J23117. This generator is constructed to detect the relationship between different promoters' strength with their swarming ability. A series of Biobricks were constructed as BBa_K2012018[https://parts.igem.org/Part:BBa_K2012018], BBa_K2012020[https://parts.igem.org/Part:BBa_K2012020] | This generator is altered from BBa_K819010 created by Peking University.Its previous promotor , J23113,is replaced by a slightly stronger promotor J23117. This generator is constructed to detect the relationship between different promoters' strength with their swarming ability. A series of Biobricks were constructed as BBa_K2012018[https://parts.igem.org/Part:BBa_K2012018], BBa_K2012020[https://parts.igem.org/Part:BBa_K2012020] | ||
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Latest revision as of 14:45, 1 November 2016
A weak CheZ generator
This generator is altered from BBa_K819010 created by Peking University.Its previous promotor , J23113,is replaced by a slightly stronger promotor J23117. This generator is constructed to detect the relationship between different promoters' strength with their swarming ability. A series of Biobricks were constructed as BBa_K2012018[1], BBa_K2012020[2]
Figure 1. The swarming plate of different E.coli K12 cheZ lacking strain, CL1, transformed with our constructed plamids.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]