Difference between revisions of "Part:BBa K1456006:Experience"
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− | </br><h2>Combination of DsbA Signal Peptide with Aprotinin to Enhance Protein Purification Lubbock_TTU iGEM 2016)</h2> | + | </br><h2>Combination of DsbA Signal Peptide with Aprotinin to Enhance Protein Purification (Lubbock_TTU iGEM 2016)</h2> |
The Aprotinin BioBrick by the ATOMS-Turkiye 2014 iGEM team was enhanced for better protein purification by using a DsbA signal tag in combination with the Aprotinin BioBrick. DsbA signal peptide carries Aprotinin to the periplasm where proper protein folding is enhanced. A protein extraction method known as osmotic shock creates pores in the outer membrane releasing the Aprotinin and other proteins in the periplasm into the supernatant. There should be fewer proteins in the solution compared with using a cell lysis method that releases all proteins within the cell into the solution. This results in cleaner protein purification of Aprotinin, while maintaining functionality. | The Aprotinin BioBrick by the ATOMS-Turkiye 2014 iGEM team was enhanced for better protein purification by using a DsbA signal tag in combination with the Aprotinin BioBrick. DsbA signal peptide carries Aprotinin to the periplasm where proper protein folding is enhanced. A protein extraction method known as osmotic shock creates pores in the outer membrane releasing the Aprotinin and other proteins in the periplasm into the supernatant. There should be fewer proteins in the solution compared with using a cell lysis method that releases all proteins within the cell into the solution. This results in cleaner protein purification of Aprotinin, while maintaining functionality. |
Latest revision as of 00:26, 31 October 2016
Aprotinin Assay
![Atoms aprotinin 3.png](/wiki/images/e/e9/Atoms_aprotinin_3.png)
Chacarterisation
![Atoms aprotinin 4.png](/wiki/images/5/5c/Atoms_aprotinin_4.png)
Combination of DsbA Signal Peptide with Aprotinin to Enhance Protein Purification (Lubbock_TTU iGEM 2016)
The Aprotinin BioBrick by the ATOMS-Turkiye 2014 iGEM team was enhanced for better protein purification by using a DsbA signal tag in combination with the Aprotinin BioBrick. DsbA signal peptide carries Aprotinin to the periplasm where proper protein folding is enhanced. A protein extraction method known as osmotic shock creates pores in the outer membrane releasing the Aprotinin and other proteins in the periplasm into the supernatant. There should be fewer proteins in the solution compared with using a cell lysis method that releases all proteins within the cell into the solution. This results in cleaner protein purification of Aprotinin, while maintaining functionality.![](https://static.igem.org/mediawiki/parts/5/5d/Lubbock_TTU_aprotinin_growth_curve.png)
Applications of BBa_K1456006
User Reviews
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