Difference between revisions of "Part:BBa K2107001"
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This Biobrick is a composite part that is made up of a stationary phase induced promoter, followed by a gene coding region for Kil protein. This part is intended to be used for in-line protein purification systems or a Kill switch. When a bacteria containing this part reaches stationary phase it will begin producing Kil protein which has been shown to degrade the outermost membrane, ultimately releasing the contents of the periplasm into the supernatant. A protein of interest could be cloned into a separate vector with a periplasm localization signal and purified from the supernatant after release by the Kil protein. This method would result in cleaner purification in comparison to traditional methods such as cell lysis. If the concentration of Kil protein reaches to high of a level it can also cause cell death. | This Biobrick is a composite part that is made up of a stationary phase induced promoter, followed by a gene coding region for Kil protein. This part is intended to be used for in-line protein purification systems or a Kill switch. When a bacteria containing this part reaches stationary phase it will begin producing Kil protein which has been shown to degrade the outermost membrane, ultimately releasing the contents of the periplasm into the supernatant. A protein of interest could be cloned into a separate vector with a periplasm localization signal and purified from the supernatant after release by the Kil protein. This method would result in cleaner purification in comparison to traditional methods such as cell lysis. If the concentration of Kil protein reaches to high of a level it can also cause cell death. | ||
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<partinfo>BBa_K2107001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2107001 SequenceAndFeatures</partinfo> | ||
Latest revision as of 00:15, 31 October 2016
Stationary Phase Promoter + Kil Protein
This Biobrick is a composite part that is made up of a stationary phase induced promoter, followed by a gene coding region for Kil protein. This part is intended to be used for in-line protein purification systems or a Kill switch. When a bacteria containing this part reaches stationary phase it will begin producing Kil protein which has been shown to degrade the outermost membrane, ultimately releasing the contents of the periplasm into the supernatant. A protein of interest could be cloned into a separate vector with a periplasm localization signal and purified from the supernatant after release by the Kil protein. This method would result in cleaner purification in comparison to traditional methods such as cell lysis. If the concentration of Kil protein reaches to high of a level it can also cause cell death.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 99
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 99
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 99
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 99
- 1000COMPATIBLE WITH RFC[1000]