Difference between revisions of "Part:BBa K2070007:Design"
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+ | Alexander A. Green, Pamela A. Silver, James J. Collins, and Peng Yin, (2014, 11) Toehold Switches: De-Novo-Designed Regulators of Gene Expression. Cell 159, 925–939. |
Latest revision as of 10:33, 30 October 2016
Toehold Trigger Inducible GFP generator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 692
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 709
Design Notes
There is no TACTAGAG sequence between toehold RBS sequence and GFP sequense.
Source
Assembly.
References
Alexander A. Green, Pamela A. Silver, James J. Collins, and Peng Yin, (2014, 11) Toehold Switches: De-Novo-Designed Regulators of Gene Expression. Cell 159, 925–939.