Difference between revisions of "Part:BBa K2179003"
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− | <h5>Translational Unit for Chlorite Dismutase | + | <h5>Translational Unit for Chlorite Dismutase Cld(-SP)</h5> |
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− | Chlorite Dismutase is an enzyme which catalyzes the reduction of chlorite ions to oxygen gas and chloride ions. Cld(-SP) is a translational unit which lacks | + | Chlorite Dismutase is an enzyme which catalyzes the reduction of chlorite ions to oxygen gas and chloride ions. Cld(-SP) is a translational unit which lacks Chlorite Dismutase original putative signal peptide, and also has a polyhistidine tag appended on it for the purpose of protein purification. The activity of this enzyme can be observed as bubbling of the liquid culture when transformed cells are combined with compounds containing the chlorite oxyanion. |
</p> | </p> | ||
<p> | <p> | ||
<partinfo>BBa_K2179003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2179003 SequenceAndFeatures</partinfo> |
Latest revision as of 23:48, 29 October 2016
Translational Unit for Chlorite Dismutase Cld(-SP)
Chlorite Dismutase is an enzyme which catalyzes the reduction of chlorite ions to oxygen gas and chloride ions. Cld(-SP) is a translational unit which lacks Chlorite Dismutase original putative signal peptide, and also has a polyhistidine tag appended on it for the purpose of protein purification. The activity of this enzyme can be observed as bubbling of the liquid culture when transformed cells are combined with compounds containing the chlorite oxyanion.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 807
Illegal BsaI.rc site found at 6