Difference between revisions of "Part:BBa K1800000"
 
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of Mambalgin Protein</h2>
 
of Mambalgin Protein</h2>
 
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This codes for the peptide Mambalgin-1, an analgesic.  Mambalgin is a protein component of the venom of Dendroaspis polylepis, better known as the Black Mamba.   Mambalgin-1 peptide that is a powerful analgesic that directly blocks pain transmission in the peripheral nervous system (Diochot et al, 2012) by targeting acid-sensing ion channels within nociceptors beneath the epidermis. Because Mambalgin acts on pain receptors within the skin rather than on opioid receptors in the brain, this peptide has great potential as a medication for pain treatment that is non-addicting and non-habit forming. Furthermore, recombinant purification of Mambalgin could assist in developing anti-venom without the attendant risk of harvesting venom directly from snakes.  This part is designed for expression in E. coli.
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Mambalgin is a potent analgesic protein found in the venom of the <i>Dendroaspis polylepis</i>  (Black Mamba snake). Mambalgin-1, the version found here, is a 3-finger toxin consisting of 57 amino acid residues forming 3 loops around a core in the shape of a hand. This protein has been found to inhibit acid-sensing ion channels (ASICs) in the central and peripheral nervous systems of mice through intraplanter and intrathecal injections. The inhibition of ASICs – important contributors to the pain pathway in both mice and humans – decreases the sensitivity of nociceptive neurons to the perception of pain. The potency of mambalgin has been compared to the drug morphine. Unlike morphine, however, mambalgin has not shown an increase in tolerance over time (Diochot et al., 2012). Furthermore, recombinant purification of Mambalgin could assist in developing anti-venom without the attendant risk of harvesting venom directly from snakes.  We  designed BBa_K1800000 part for expression in E.<i>coli</i>.
  
 
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1800000 SequenceAndFeatures</partinfo>
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Latest revision as of 07:55, 29 October 2016


Mambalgin-1 for E. coli BBa_ K1800000

Brief introduction of Mambalgin Protein

Mambalgin is a potent analgesic protein found in the venom of the Dendroaspis polylepis (Black Mamba snake). Mambalgin-1, the version found here, is a 3-finger toxin consisting of 57 amino acid residues forming 3 loops around a core in the shape of a hand. This protein has been found to inhibit acid-sensing ion channels (ASICs) in the central and peripheral nervous systems of mice through intraplanter and intrathecal injections. The inhibition of ASICs – important contributors to the pain pathway in both mice and humans – decreases the sensitivity of nociceptive neurons to the perception of pain. The potency of mambalgin has been compared to the drug morphine. Unlike morphine, however, mambalgin has not shown an increase in tolerance over time (Diochot et al., 2012). Furthermore, recombinant purification of Mambalgin could assist in developing anti-venom without the attendant risk of harvesting venom directly from snakes. We designed BBa_K1800000 part for expression in E.coli.