Difference between revisions of "Part:BBa K1893006"
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The Cin receiver device consists of a constitutively expressed CinR transcription regulator [https://parts.igem.org/Part:BBa_K1893028 (BBa_K1893028)] upstream of a cognate pCin promoter [https://parts.igem.org/Part:BBa_K1893029 (BBa_K1893029)]. CinR is activated in the presence of a O3-C14 HSL signal and binds to pCin to initiate transcription of a downstream coding sequence. | The Cin receiver device consists of a constitutively expressed CinR transcription regulator [https://parts.igem.org/Part:BBa_K1893028 (BBa_K1893028)] upstream of a cognate pCin promoter [https://parts.igem.org/Part:BBa_K1893029 (BBa_K1893029)]. CinR is activated in the presence of a O3-C14 HSL signal and binds to pCin to initiate transcription of a downstream coding sequence. | ||
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===Usage and biology=== | ===Usage and biology=== | ||
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In this case, the constitutively expressed CinR transcriptional regulator [https://parts.igem.org/Part:BBa_K1893028 (BBa_K1893028)] is activated by the binding of O3-C14 HSL, an AHL quorum signal. The activated CinR regulator binds to the CinR-inducible promoter, pCin, to initiate transcription of a downstream gene. | In this case, the constitutively expressed CinR transcriptional regulator [https://parts.igem.org/Part:BBa_K1893028 (BBa_K1893028)] is activated by the binding of O3-C14 HSL, an AHL quorum signal. The activated CinR regulator binds to the CinR-inducible promoter, pCin, to initiate transcription of a downstream gene. | ||
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===Characterisation=== | ===Characterisation=== | ||
We characterised the activation range of this device using a GFP reporter. The results of our characterisation experiments can be found here [https://parts.igem.org/Part:BBa_K1893007 (BBa_K1893007)] | We characterised the activation range of this device using a GFP reporter. The results of our characterisation experiments can be found here [https://parts.igem.org/Part:BBa_K1893007 (BBa_K1893007)] | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 03:20, 29 October 2016
Cin receiver (CinR+pCin)
The Cin receiver device consists of a constitutively expressed CinR transcription regulator (BBa_K1893028) upstream of a cognate pCin promoter (BBa_K1893029). CinR is activated in the presence of a O3-C14 HSL signal and binds to pCin to initiate transcription of a downstream coding sequence.
Usage and biology
The Cin receiver is based on the Cin quorum-sensing system from Rhizobium leguminosarum. Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression.
In this case, the constitutively expressed CinR transcriptional regulator (BBa_K1893028) is activated by the binding of O3-C14 HSL, an AHL quorum signal. The activated CinR regulator binds to the CinR-inducible promoter, pCin, to initiate transcription of a downstream gene.
Characterisation
We characterised the activation range of this device using a GFP reporter. The results of our characterisation experiments can be found here (BBa_K1893007)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 611
- 1000COMPATIBLE WITH RFC[1000]