Difference between revisions of "Part:BBa K2066028:Experience"
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William and Mary iGEM 2016 sequence confirmed this part. | William and Mary iGEM 2016 sequence confirmed this part. | ||
| − | We also validated its fluorescence and repressor functionality (in combination with a pLac GFP reporter BBa_K2066014) through flow cytometry. | + | We also validated its fluorescence, induction capability, and repressor functionality (in combination with a pLac GFP reporter BBa_K2066014) through flow cytometry. |
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| + | Validation of fluorescence and induction capability: | ||
https://static.igem.org/mediawiki/parts/9/97/William_and_Mary_28_IC3_Induction.png | https://static.igem.org/mediawiki/parts/9/97/William_and_Mary_28_IC3_Induction.png | ||
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| + | We did not obtain plots for repressor functionality due to a data storage error, but we did confirm that with fixed Arabinose concentration at 1 mM and variable IPTG induction over 0 - 10 mM range, that FL3 fluorescence levels (to detect mCherry) were were constant across the induction (in arbitrary fluorescence units) while the FL1 fluorescence levels (to detect sfGFP from BBa_K2066014) increased with induction (in arbitrary fluorescence units). | ||
===Applications of BBa_K2066028=== | ===Applications of BBa_K2066028=== | ||
Latest revision as of 03:18, 29 October 2016
William and Mary iGEM 2016 sequence confirmed this part.
We also validated its fluorescence, induction capability, and repressor functionality (in combination with a pLac GFP reporter BBa_K2066014) through flow cytometry.
Validation of fluorescence and induction capability:
We did not obtain plots for repressor functionality due to a data storage error, but we did confirm that with fixed Arabinose concentration at 1 mM and variable IPTG induction over 0 - 10 mM range, that FL3 fluorescence levels (to detect mCherry) were were constant across the induction (in arbitrary fluorescence units) while the FL1 fluorescence levels (to detect sfGFP from BBa_K2066014) increased with induction (in arbitrary fluorescence units).
Applications of BBa_K2066028
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