Difference between revisions of "Part:BBa K2066037"

Latest revision as of 04:50, 29 October 2016

pACT-Tet on UNS Standard

This is the pACT-Tet sequence from Amit et al. 2011 “Building Synthetic Enhancers from the Ground Up” paper on the UNS standard. This part consists of the endogenous nitrogen regulatory promoter, the NRII mutant BBa_K2066112 and tetR. This is meant for use in Tet based synthetic enhancer systems, such as the [http://2016.igem.org/Team:William_and_Mary/Synthetic_Enhancer William and Mary 2016]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 969
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 2343
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2066037 short</partinfo>
-This is the pACT-Tet sequence from Amit et al. 2011 “Building Synthetic Enhancers from the Ground Up” paper on the UNS standard. This part consists of the endogenous nitrogen regulatory promoter, the NRII mutant  BBa_K20660112 and tetR.  This is meant for use in Tet based synthetic enhancer systems, such as the [http://2016.igem.org/Team:William_and_Mary/Synthetic_Enhancer William and Mary 2016]
+This is the pACT-Tet sequence from Amit et al. 2011 “Building Synthetic Enhancers from the Ground Up” paper on the UNS standard. This part consists of the endogenous nitrogen regulatory promoter, the NRII mutant  BBa_K2066112 and tetR.  This is meant for use in Tet based synthetic enhancer systems, such as the [http://2016.igem.org/Team:William_and_Mary/Synthetic_Enhancer William and Mary 2016]
-Note this part does not have a terminator, as the plasmid it was derived from used the terminator of the origin of replication.
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