Difference between revisions of "Part:BBa K2109109"
 
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HDVR U1A is a non-functional ribozyme negative control. It can be in vitro transcribed via T7-RNA Polymerase to produce a transcript of approximately ~250 bp. The other HDVR control U1A-C75T (Part:BBa_K2109110)is inducible in the presence of 1% xylose and also produces of transcript of ~250 bp when in vitro transcribed. Ribozyme sequence obtained from (doi:10.1038) and was modified to conform to bio-brick standard.
 
HDVR U1A is a non-functional ribozyme negative control. It can be in vitro transcribed via T7-RNA Polymerase to produce a transcript of approximately ~250 bp. The other HDVR control U1A-C75T (Part:BBa_K2109110)is inducible in the presence of 1% xylose and also produces of transcript of ~250 bp when in vitro transcribed. Ribozyme sequence obtained from (doi:10.1038) and was modified to conform to bio-brick standard.
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Latest revision as of 00:05, 29 October 2016


HDVR U1A gBlock

HDVR U1A is a non-functional ribozyme negative control. It can be in vitro transcribed via T7-RNA Polymerase to produce a transcript of approximately ~250 bp. The other HDVR control U1A-C75T (Part:BBa_K2109110)is inducible in the presence of 1% xylose and also produces of transcript of ~250 bp when in vitro transcribed. Ribozyme sequence obtained from (doi:10.1038) and was modified to conform to bio-brick standard.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 58
    Illegal NgoMIV site found at 87
  • 1000
    COMPATIBLE WITH RFC[1000]