Difference between revisions of "Part:BBa M50021:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | All parts are optimized for expression in E. coli. Terminator was added to prevent expression of the GFP gene included in the plasmid this construct was going to be inserted into the E. coli promoter cassette plasmid provided by DNA 2.0. | |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | RBS and terminator are derived from bacteriophage T7; sequences provided by GeneDesigner software | |
| − | + | Promoter comes from groE gene in E. coli | |
| − | + | d-limonene synthase gene was sequenced from Citrus unshiu, then optimized for E. coli | |
===References=== | ===References=== | ||
| + | GeneDesigner | ||
| + | Wang & deHaseth, 2003 (promoter) | ||
| + | Lindler, 1994 (promoter) | ||
| + | Shimada, 2005 (d-limonene synthase) | ||
Latest revision as of 01:43, 12 December 2016
Heat-inducible d-Limonene synthase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1895
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1600
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1597
Illegal SapI.rc site found at 319
Illegal SapI.rc site found at 1572
Design Notes
All parts are optimized for expression in E. coli. Terminator was added to prevent expression of the GFP gene included in the plasmid this construct was going to be inserted into the E. coli promoter cassette plasmid provided by DNA 2.0.
Source
RBS and terminator are derived from bacteriophage T7; sequences provided by GeneDesigner software Promoter comes from groE gene in E. coli d-limonene synthase gene was sequenced from Citrus unshiu, then optimized for E. coli
References
GeneDesigner Wang & deHaseth, 2003 (promoter) Lindler, 1994 (promoter) Shimada, 2005 (d-limonene synthase)