Difference between revisions of "Part:BBa K1893023"
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This is the Anti-STAR sequence under control of the Rhl quorum signalling system. The gene encoding the transcriptional activator RhlR is downstream a constitutive Anderson promoter, j23101. RhlR is activated by C4-HSL. Included in the construct is the RhlR-activated promoter pRhl. | This is the Anti-STAR sequence under control of the Rhl quorum signalling system. The gene encoding the transcriptional activator RhlR is downstream a constitutive Anderson promoter, j23101. RhlR is activated by C4-HSL. Included in the construct is the RhlR-activated promoter pRhl. | ||
Latest revision as of 15:14, 27 October 2016
Rhl receiver with anti-STAR (RhlR+pRhl+anti-STAR)
This is the Anti-STAR sequence under control of the Rhl quorum signalling system. The gene encoding the transcriptional activator RhlR is downstream a constitutive Anderson promoter, j23101. RhlR is activated by C4-HSL. Included in the construct is the RhlR-activated promoter pRhl.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 301
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 776