Difference between revisions of "Part:BBa K1893015:Design"

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===Design Notes===
 
===Design Notes===
  
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The reverse terminator [https://parts.igem.org/Part:BBa_B0025 BBa_B0025] was added to part [https://parts.igem.org/Part:BBa_K1321333 BBa_K1321333], for use in the pSB1A2 plasmid. This was done because a reverse terminator is present in the pSB1C3 plasmid, but not in pSB1A2
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We added a reverse terminator to the original pBAD promoter system in order to terminate translation of the araC coding sequence in plasmid backbones that lack an upstream terminator, such as pSB1A2.
  
 
===Source===
 
===Source===
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This part was created by ligating the araC and pBAD inducible promoter construct in the distribution kit (BBa_K1321333) with  the BBa_B0025 reverse terminator
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This part was created by ligating the araC and pBAD inducible promoter construct in the distribution kit [https://parts.igem.org/Part:BBa_K1321333 (BBa_K1321333)] with  the [https://parts.igem.org/Part:BBa_B0025 BBa_B0025] reverse terminator
  
 
===References===
 
===References===

Latest revision as of 20:00, 28 October 2016


Arabinose inducible promoter (pBAD)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1342
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1281
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1116
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1098


Design Notes

We added a reverse terminator to the original pBAD promoter system in order to terminate translation of the araC coding sequence in plasmid backbones that lack an upstream terminator, such as pSB1A2.

Source

This part was created by ligating the araC and pBAD inducible promoter construct in the distribution kit (BBa_K1321333) with the BBa_B0025 reverse terminator

References