Difference between revisions of "Part:BBa K209472"

Latest revision as of 05:13, 27 October 2016

AarI B-D part, ADRA2A (B2AR)

This is the coding sequence of Homo sapiens adrenoceptor beta 2 (ADRB2). This part has been improved by SJTU-Biox-Shanghai 2016 iGEM team to generate two parts with modified sequence: BBa_K1969001 and BBa_K1969002.

BBa_K1969001 Group: SJTU-Biox-Shanghai 2016 Author: Yijie Lai Summary: This improved version using site mutation to eliminate the illegal PstI site by changing G at 192 bp to A, and was tested for epinephrine response when expressed in yeast Saccharomyces cerevisiae under two different promoters, GAL1 and ADH1.

BBa_K1969002 Group: SJTU-Biox-Shanghai 2016 Author: Yijie Lai Summary: This improved version using site mutation to eliminate the illegal PstI site by changing G at 192 bp to A and adding a his-tag to its C terminus. The sequence was tested for membrane localization when expressed in yeast Saccharomyces cerevisiae under two different promoters, GAL1 and ADH1.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 190
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 190
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 778
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 190
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 190
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 364
Illegal BsaI.rc site found at 562

@@ Line 9: / Line 9: @@
 Author: Yijie Lai
 Summary: This improved version using site  mutation to eliminate the illegal PstI site by changing G at 192 bp to A, and was tested for epinephrine response when expressed in yeast Saccharomyces cerevisiae under two different promoters, GAL1 and ADH1.
-Uploads:
 BBa_K1969002
@@ Line 15: / Line 14: @@
 Author: Yijie Lai
 Summary: This improved version using site  mutation to eliminate the illegal PstI site by changing G at 192 bp to A and adding a his-tag to its C terminus. The sequence was tested for membrane localization when expressed in yeast Saccharomyces cerevisiae under two different promoters, GAL1 and ADH1.
-Uploads: