Difference between revisions of "Part:BBa K1893016:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | Gp2 was characterised using an arabinose inducible promoter, because constitutive expression, and the resultant arrest of E. coli growth, would result in several potential problems in the cloning and characterisation workflow. The sequence for Gp2 was obtained from the NCBI, and codon optimisation was not required, as the protein is from en E. coli phage. | ||
===Source=== | ===Source=== | ||
| − | Gp2 was synthesised with a ribosome binding site using the ThermoFisher GeneArt service. This basic part (BBa_K1893019) was ligated in front of our pBAD construct BBa_K1893015 | + | Gp2 was synthesised with a ribosome binding site using the ThermoFisher GeneArt service. This basic part [https://parts.igem.org/Part:BBa_K1893019 (BBa_K1893019)] was ligated in front of our pBAD construct [https://parts.igem.org/Part:BBa_K1893015 BBa_K1893015] |
===References=== | ===References=== | ||
Latest revision as of 14:53, 27 October 2016
Arabinose inducible gp2 (pBAD+gp2)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1342
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1281
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1116
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1473
Illegal SapI site found at 1098
Design Notes
Gp2 was characterised using an arabinose inducible promoter, because constitutive expression, and the resultant arrest of E. coli growth, would result in several potential problems in the cloning and characterisation workflow. The sequence for Gp2 was obtained from the NCBI, and codon optimisation was not required, as the protein is from en E. coli phage.
Source
Gp2 was synthesised with a ribosome binding site using the ThermoFisher GeneArt service. This basic part (BBa_K1893019) was ligated in front of our pBAD construct BBa_K1893015