Difference between revisions of "Part:BBa K1878004:Experience"
 
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===Applications of BBa_K1878004===
 
===Applications of BBa_K1878004===
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<I>Rice iGEM 2016</I>
 
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For the 2016 Team Rice project, we built a biosensor construct in <i>E. coli</i> with fdhF and iRFP670 to fluoresce in the absence of oxygen. Specifically, formate is a metabolite that is generated in E.coli under anaerobic conditions, which binds to fdhA to induce transcription downstream of fdhF.
 
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[[Image:Fdhf_assay_670.jpg|thumb|left|800px|Fig 1. Results of part assay.]]
 
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===User Reviews===
 
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<partinfo>BBa_K1878004 AddReview number</partinfo>
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<I>Rice iGEM 2016</I>
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For the 2016 Team Rice project, we built a biosensor construct in <i>E. coli</i> with fdhF and iRFP670 to fluoresce in the absence of oxygen. Specifically, the cofactor fdhA binds to formate, a metabolite in anaerobic respiration, to upregulate transcription downstream of fdhF. However, despite this description from literature, our assay results indicate that transcription was downregulated in anaerobic conditions.
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[[Image:Fdhf_assay_670.jpg|thumb|left|800px|Fig 1. Results of part assay in aerobic, and anaerobic, conditions.]]
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Latest revision as of 07:33, 26 October 2016


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Applications of BBa_K1878004

User Reviews

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No review score entered. Rice iGEM 2016

For the 2016 Team Rice project, we built a biosensor construct in E. coli with fdhF and iRFP670 to fluoresce in the absence of oxygen. Specifically, the cofactor fdhA binds to formate, a metabolite in anaerobic respiration, to upregulate transcription downstream of fdhF. However, despite this description from literature, our assay results indicate that transcription was downregulated in anaerobic conditions.

Fig 1. Results of part assay in aerobic, and anaerobic, conditions.