Difference between revisions of "Part:BBa K1878005:Experience"
 
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<I>Rice iGEM 2016</I>
 
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For the 2016 Team Rice project, we built a biosensor construct in <i>E. coli</i> between fdhF and iRFP713 to fluoresce in the absence of oxygen. Specifically, formate is a metabolite that is generated in E.coli under anaerobic conditions, which binds to fdhA to induce transcription downstream of fdhF.
 
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[[Image:FdhF_iRFP713_Hypoxia_Assay.jpeg|thumb|left|800px|Fig 1. Results of part assay.]]
 
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<I>Rice iGEM 2016</I>
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For the 2016 Team Rice project, we built a biosensor construct in <i>E. coli</i> with fdhF and iRFP713 to fluoresce in the absence of oxygen. Specifically, the cofactor fdhA binds to formate, a metabolite in anaerobic respiration, to upregulate transcription downstream of fdhF. However, despite this description from literature, our assay results indicate that transcription was downregulated in anaerobic conditions.
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[[Image:FdhF_iRFP713_Hypoxia_Assay.jpeg|thumb|left|800px|Fig 1. Results of part assay in aerobic, and anaerobic, conditions.]]
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Latest revision as of 07:36, 26 October 2016


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No review score entered. Rice iGEM 2016

For the 2016 Team Rice project, we built a biosensor construct in E. coli with fdhF and iRFP713 to fluoresce in the absence of oxygen. Specifically, the cofactor fdhA binds to formate, a metabolite in anaerobic respiration, to upregulate transcription downstream of fdhF. However, despite this description from literature, our assay results indicate that transcription was downregulated in anaerobic conditions.

Fig 1. Results of part assay in aerobic, and anaerobic, conditions.