Difference between revisions of "Part:BBa K1878006:Experience"
 
(10 intermediate revisions by 2 users not shown)
Line 7: Line 7:
  
 
===User Reviews===
 
===User Reviews===
−
2016 Rice
 
 
<!-- DON'T DELETE --><partinfo>BBa_K1878006 StartReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K1878006 StartReviews</partinfo>
 
<!-- Template for a user review
 
<!-- Template for a user review
Line 20: Line 19:
 
<!-- End of the user review template -->
 
<!-- End of the user review template -->
 
<!-- DON'T DELETE --><partinfo>BBa_K1878006 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K1878006 EndReviews</partinfo>
 +
 +
{|width='80%' style='border:1px solid gray'
 +
|-
 +
|width='10%'|
 +
<partinfo>BBa_K1878006 AddReview number</partinfo>
 +
<I>Rice iGEM 2016</I>
 +
|width='60%' valign='top'|
 +
For the 2016 Team Rice project, we built a biosensor in <i>E. coli</i> with a modified version of NarK (mNarK from 2009 UCL London's BBa_K239006) and iRFP670 to fluoresce in anaerobic conditions. Despite literature description that NarK should be upregulated in anaerobic conditions, the <strong><em>results of our characterization indicate that transcription is downregulated</em></strong>.
 +
[[Image:MNarK_IRFP670_Hypoxia_Assay.jpg|thumb|left|800px|Fig 1. Results of part assay (construct output iRFP670) in aerobic, and anaerobic conditions -- oxygen was purged with nitrogen gas. Results are averaged over 8 samples that ran simultaneously in a single experiment.]]
 +
|}

Latest revision as of 03:32, 27 October 2016


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1878006

User Reviews

UNIQ469fad9c1afb8aad-partinfo-00000000-QINU UNIQ469fad9c1afb8aad-partinfo-00000001-QINU

No review score entered. Rice iGEM 2016

For the 2016 Team Rice project, we built a biosensor in E. coli with a modified version of NarK (mNarK from 2009 UCL London's BBa_K239006) and iRFP670 to fluoresce in anaerobic conditions. Despite literature description that NarK should be upregulated in anaerobic conditions, the results of our characterization indicate that transcription is downregulated.

Fig 1. Results of part assay (construct output iRFP670) in aerobic, and anaerobic conditions -- oxygen was purged with nitrogen gas. Results are averaged over 8 samples that ran simultaneously in a single experiment.