Difference between revisions of "Part:BBa K1893005"
 
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<partinfo>BBa_K1893005 short</partinfo>
 
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The gene encoding the transcriptional activator LuxR is downstream a constitutive Anderson promoter, j23101. LuxR is activated by O3C6-HSL. Included in the construct is the LuxR-activated promoter pLux upstream a reporter sequence which includes GFP. This part was designed so that we could characterize the LuxR quorum sensing system and determine activation ranges and crosstalk data.
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This composite part is based on the Lux quorum-sensing system from Vibrio fischerii. It consists of a Lux receiver device and a GFP reporter that is activated in the presence of a 3O-C6 AHL signal.
  
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===Usage and Biology===
 
===Usage and Biology===
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Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression.
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In this case, the constitutively expressed LuxR transcriptional regulator (C0062)  is activated by the binding of 3O-C6 AHL, an AHL quorum signal. The activated LuxR regulator binds to a LuxR-inducible promoter, pLux, upstream of a GFP reporter. As a result, GFP is expressed when the receiver device is induced with 3O-C6 AHL. We designed this part to characterize the activation range of the Lux receiver device. We are also currently characterising the cross-talk of the Lux receiver device with other quorum-sensing systems, in order to determine its orthogonality.
  
 
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Latest revision as of 21:40, 28 October 2016


Lux receiver with GFP (LuxR+pLux+GFP)

This composite part is based on the Lux quorum-sensing system from Vibrio fischerii. It consists of a Lux receiver device and a GFP reporter that is activated in the presence of a 3O-C6 AHL signal.

Usage and Biology

Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression.

In this case, the constitutively expressed LuxR transcriptional regulator (C0062) is activated by the binding of 3O-C6 AHL, an AHL quorum signal. The activated LuxR regulator binds to a LuxR-inducible promoter, pLux, upstream of a GFP reporter. As a result, GFP is expressed when the receiver device is induced with 3O-C6 AHL. We designed this part to characterize the activation range of the Lux receiver device. We are also currently characterising the cross-talk of the Lux receiver device with other quorum-sensing systems, in order to determine its orthogonality.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985
    Illegal BsaI.rc site found at 1712