Difference between revisions of "Part:BBa K1132010"
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<partinfo>BBa_K1132010 short</partinfo> | <partinfo>BBa_K1132010 short</partinfo> | ||
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The site-specific recombination system of temperate lactococcal bacteriophage TP901-1 integrase mediates site-specific recombination system. Originally from temperate lactoccocal bacteriophage TP901-1, this is a serine-type integrase able to invert, integrate or excise a DNA fragment according to the position and orientation of its specific recognition sites, attB and attP. This process is directional and definitive because of the transformation of attB and attP into attL and attR during recombination. | The site-specific recombination system of temperate lactococcal bacteriophage TP901-1 integrase mediates site-specific recombination system. Originally from temperate lactoccocal bacteriophage TP901-1, this is a serine-type integrase able to invert, integrate or excise a DNA fragment according to the position and orientation of its specific recognition sites, attB and attP. This process is directional and definitive because of the transformation of attB and attP into attL and attR during recombination. | ||
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<br><br><center>attB + attP + integrase → attR + attL + integrase</center><br><br> | <br><br><center>attB + attP + integrase → attR + attL + integrase</center><br><br> | ||
https://static.igem.org/mediawiki/2013/3/37/TP901.png | https://static.igem.org/mediawiki/2013/3/37/TP901.png | ||
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+ | <h1>Improvement by iGEM Peking 2017</h1> | ||
+ | This part has been improved by iGEM Peking 2017. | ||
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+ | [[Part:BBa_K2243000|Click here to get more information about Part:BBa_K2243000!]] | ||
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<li>'''Author:''' Lukas Schmidheini | <li>'''Author:''' Lukas Schmidheini | ||
<li>'''Summary:''' We cloned and characterised a codon optimised TP901-1 for <i>E. coli</i>, and sent it to the registry as | <li>'''Summary:''' We cloned and characterised a codon optimised TP901-1 for <i>E. coli</i>, and sent it to the registry as | ||
− | a biobrick. Our biobrick can be found with a degradation tag [[Part: BBa_K2116010]] and without any degradation | + | a biobrick. Our biobrick can be found with a degradation tag [[Part:BBa_K2116010]] and without any degradation |
tag [[Part:BBa_K2116035]]. The non-codon optimised version can be found here [[Part:BBa K1132010]]. Besides some favorable features, the codon optimized TP901-1 also supports full RFC[25] assembly and is available under the Tet promoter [[Part:BBa_R0040]]: TP90-1: [[Part:BBa_K2116064]], TP901-1 with ssrA tag: [[Part:BBa_K2116062]]. | tag [[Part:BBa_K2116035]]. The non-codon optimised version can be found here [[Part:BBa K1132010]]. Besides some favorable features, the codon optimized TP901-1 also supports full RFC[25] assembly and is available under the Tet promoter [[Part:BBa_R0040]]: TP90-1: [[Part:BBa_K2116064]], TP901-1 with ssrA tag: [[Part:BBa_K2116062]]. | ||
Latest revision as of 14:48, 29 October 2017
Tp901 integrase
The site-specific recombination system of temperate lactococcal bacteriophage TP901-1 integrase mediates site-specific recombination system. Originally from temperate lactoccocal bacteriophage TP901-1, this is a serine-type integrase able to invert, integrate or excise a DNA fragment according to the position and orientation of its specific recognition sites, attB and attP. This process is directional and definitive because of the transformation of attB and attP into attL and attR during recombination.
The 180° switch permits to design a lot of regulation tools, such as logical gates that can be found here (https://parts.igem.org/Part:BBa_K1132001, https://parts.igem.org/Part:BBa_K1132002).
Improvement by iGEM Peking 2017
This part has been improved by iGEM Peking 2017.
Click here to get more information about Part:BBa_K2243000!
Contribution by ETH Zurich 2016
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 28
Illegal AgeI site found at 1486 - 1000COMPATIBLE WITH RFC[1000]