Difference between revisions of "Part:BBa I732094"

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<partinfo>BBa_I732401 short</partinfo>
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<partinfo>BBa_I732094 short</partinfo>
  
A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB1A3.
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Promoter can be inserted into the adapter containing BglII and BamHI sites. There are two downstream reporters, LacZ-alpha and GFP-AAV. The activity of inserted promoter can be qualitatively observed on a X-gal plate, or be quantificationally measured using ONPG assay or fluorescent assay.
 
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For more information please refer to BBa_I732401.
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* It's named as DPv2A as on pSB1A3 plasmid.
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* It's named as DPv2D as on pSB3K5 plasmid.
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* It's named as DPv2L as on pSB3C5 plasmid.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_I732401 SequenceAndFeatures</partinfo>
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<partinfo>BBa_I732094 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
<partinfo>BBa_I732401 parameters</partinfo>
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<partinfo>BBa_I732094 parameters</partinfo>
 
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Latest revision as of 14:38, 14 October 2007

Promoter Activity Reporter (LacZ-alpha and GFP-AAV)

Promoter can be inserted into the adapter containing BglII and BamHI sites. There are two downstream reporters, LacZ-alpha and GFP-AAV. The activity of inserted promoter can be qualitatively observed on a X-gal plate, or be quantificationally measured using ONPG assay or fluorescent assay.

  • It's named as DPv2A as on pSB1A3 plasmid.
  • It's named as DPv2D as on pSB3K5 plasmid.
  • It's named as DPv2L as on pSB3C5 plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3
    Illegal BamHI site found at 12
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 949