Difference between revisions of "Part:BBa K2120418:Experience"
 
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<br>We have linked this circuit in pSB3K3 and pSB4K5 and tested them in ''E.coli BMTop10''. We cultured our bacteria overnight and then tested fluorescence intensity to indicate the expression level of circuit in different plasmids.
 
<br>We have linked this circuit in pSB3K3 and pSB4K5 and tested them in ''E.coli BMTop10''. We cultured our bacteria overnight and then tested fluorescence intensity to indicate the expression level of circuit in different plasmids.
 
<br>The fluorescence intensity is shown in Fig.1. As,we all know, the copy number of pSB3K3 is little higher than the copy number of pSB4K5. And according to the result, the fluorescence intensity of bacteria containing pSB4K5 is higher than containing pSB3K3. It means our system is working. Our killer would express more with less plasmids.
 
<br>The fluorescence intensity is shown in Fig.1. As,we all know, the copy number of pSB3K3 is little higher than the copy number of pSB4K5. And according to the result, the fluorescence intensity of bacteria containing pSB4K5 is higher than containing pSB3K3. It means our system is working. Our killer would express more with less plasmids.
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[[File:BIT-CHINA-PARTS-INHIBITOR-20.jpg|thumb|Fig1. The intensity of fluorescence is detected in two kinds of plasmids with different copy number.|center]]
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[[File:BIT-CHINA-PARTS-INHIBITOR-20.jpg|thumb|Fig1. The intensity of fluorescence is detected in two kinds of plasmids with different copy number.|600px|center]]
 
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Latest revision as of 17:00, 23 October 2016


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[http://2016.igem.org/Team:BIT-China BIT-China iGEM 2016 Team]


We have linked this circuit in pSB3K3 and pSB4K5 and tested them in E.coli BMTop10. We cultured our bacteria overnight and then tested fluorescence intensity to indicate the expression level of circuit in different plasmids.
The fluorescence intensity is shown in Fig.1. As,we all know, the copy number of pSB3K3 is little higher than the copy number of pSB4K5. And according to the result, the fluorescence intensity of bacteria containing pSB4K5 is higher than containing pSB3K3. It means our system is working. Our killer would express more with less plasmids.

Fig1. The intensity of fluorescence is detected in two kinds of plasmids with different copy number.
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