Difference between revisions of "Part:BBa K1893014:Design"
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===Source=== | ===Source=== | ||
| − | Anti-STAR is a sequence that Imperial iGEM 2016 have designed for the first time. | + | Anti-STAR is a sequence that Imperial iGEM 2016 have designed for the first time using the [http://rna.urmc.rochester.edu/RNAstructure.html RNAstructure] software. |
===References=== | ===References=== | ||
Imperial iGEM 2016 | Imperial iGEM 2016 | ||
Latest revision as of 15:35, 27 October 2016
anti-STAR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence was obtained through the use of inverse PCR on the 'j23119-AntiSTAR/pSB1C3' construct (BBa_K1893011) to remove the constitutive promoter j23119.
Anti-STAR forward primer: AGTTTTTACAGTGAATTGTTTTAATTAGTTG
Prefix Reverse primer: CTCTAGAAGCGGCCGCG
Source
Anti-STAR is a sequence that Imperial iGEM 2016 have designed for the first time using the [http://rna.urmc.rochester.edu/RNAstructure.html RNAstructure] software.
References
Imperial iGEM 2016