Difference between revisions of "Part:BBa K1893009:Design"

(Chappell, J., Takahashi, M. & Lucks, J. (2015) Creating small transcription activating RNAs. Nature Chemical Biology. [Online] 11 (3), 214-220)
 
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<partinfo>BBa_K1893009 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1893009 SequenceAndFeatures</partinfo>
  
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===Design Notes===
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This sequence was synthesised as a block.
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This sequence was synthesised as a block with BioBrick Prefix on the 5' end and BioBrick Suffix on the 3' end.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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Chappell, J., Takahashi, M. & Lucks, J. (2015) Creating small transcription activating RNAs. Nature Chemical Biology. [Online] 11 (3), 214-220

Latest revision as of 11:11, 23 October 2016


STAR inducible superfolder GFP (pAD1.S5+SF-GFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 569
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 959
    Illegal BsaI site found at 1137

Design Notes

This sequence was synthesised as a block with BioBrick Prefix on the 5' end and BioBrick Suffix on the 3' end.

Source

The pAD1.S5 attenuator sequence (STAR target)

References

Chappell, J., Takahashi, M. & Lucks, J. (2015) Creating small transcription activating RNAs. Nature Chemical Biology. [Online] 11 (3), 214-220