Difference between revisions of "Part:BBa K1893008"
 
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<partinfo>BBa_K1893008 short</partinfo>
 
<partinfo>BBa_K1893008 short</partinfo>
  
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This is a construct for constitutively expressing RhlI gene. RhlI is a protein that synthesises N-butyryl-HSL, which activates the transcriptional activator RhlR.
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The Rhl sender device consists of a constitutively expressed RhlI AHL synthase [https://parts.igem.org/Part:BBa_C0170 (BBa_C0170)] under the control of a moderate Anderson promoter [https://parts.igem.org/Part:BBa_J23105 (BBa_J23105)]. RhlI produces the C4-AHL signal that is required for activation of the RhlR transcription regulator.
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===Usage and biology===
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The Rhl sender is based on the Rhl quorum-sensing system from <i>Pseudomonas aeruginosa</i>. Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression.
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The constitutively expressed RhlI AHL synthesis the C4-AHL signal that binds to RhlR to activate the transcription regulator. Activated RhlR can then bind to the RhlR-inducible promoter, pRhl, in order to initiate transcription of a downstream coding sequence.
  
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===Usage and Biology===
 
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1893008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1893008 SequenceAndFeatures</partinfo>

Latest revision as of 03:22, 29 October 2016


Constitutive Rhl sender (J23105+RhlI)

The Rhl sender device consists of a constitutively expressed RhlI AHL synthase (BBa_C0170) under the control of a moderate Anderson promoter (BBa_J23105). RhlI produces the C4-AHL signal that is required for activation of the RhlR transcription regulator.


Usage and biology

The Rhl sender is based on the Rhl quorum-sensing system from Pseudomonas aeruginosa. Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression.

The constitutively expressed RhlI AHL synthesis the C4-AHL signal that binds to RhlR to activate the transcription regulator. Activated RhlR can then bind to the RhlR-inducible promoter, pRhl, in order to initiate transcription of a downstream coding sequence.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]