Difference between revisions of "Part:BBa K2120301"
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This device is composed of induced promoter pR , ''cI'' ([https://parts.igem.org/wiki/index.php?title=Part:BBa_C0051 BBa_C0051]), and a composite part J133000([https://parts.igem.org/wiki/index.php?title=Part:BBa_J133000 BBa_J133000]). ''RFP'' is employed as report gene. The pR promoter can be repressed by its inhibitor ''cI''. The expression of RFP will decrease corresponding the increasing of inhibitor to indicate the switch’s expression level. | This device is composed of induced promoter pR , ''cI'' ([https://parts.igem.org/wiki/index.php?title=Part:BBa_C0051 BBa_C0051]), and a composite part J133000([https://parts.igem.org/wiki/index.php?title=Part:BBa_J133000 BBa_J133000]). ''RFP'' is employed as report gene. The pR promoter can be repressed by its inhibitor ''cI''. The expression of RFP will decrease corresponding the increasing of inhibitor to indicate the switch’s expression level. | ||
− | This device is designed to construct K2120310([https://parts.igem.org/wiki/index.php?title=Part: | + | This device is designed to construct K2120310([https://parts.igem.org/wiki/index.php?title=Part:BBa_K2120310 BBa_K2120310]). |
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Latest revision as of 15:21, 22 October 2016
pR+B0032+RFP+B0015
This device is composed of induced promoter pR , cI (BBa_C0051), and a composite part J133000(BBa_J133000). RFP is employed as report gene. The pR promoter can be repressed by its inhibitor cI. The expression of RFP will decrease corresponding the increasing of inhibitor to indicate the switch’s expression level. This device is designed to construct K2120310(BBa_K2120310).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 631
Illegal AgeI site found at 743 - 1000COMPATIBLE WITH RFC[1000]