Difference between revisions of "Part:BBa K1983015:Design"
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This part has an XbaI site between RBS ([https://parts.igem.org/Part:BBa_J61132 J61132]) and PheP gene ([https://parts.igem.org/Part:BBa_K1983013 K1983013]) because it was created by fusing ([https://parts.igem.org/Part:BBa_K1983014 K1983014]) with ([https://parts.igem.org/Part:BBa_K1983011 K1983011]). <br>
 
This part has an XbaI site between RBS ([https://parts.igem.org/Part:BBa_J61132 J61132]) and PheP gene ([https://parts.igem.org/Part:BBa_K1983013 K1983013]) because it was created by fusing ([https://parts.igem.org/Part:BBa_K1983014 K1983014]) with ([https://parts.igem.org/Part:BBa_K1983011 K1983011]). <br>
  
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Since one of the fused parts is a composite biobrick ([https://parts.igem.org/Part:BBa_K1983015 K1983015]) comprised of a promoter, RBS, coding gene and terminator, with XbaI it is more adaptable regarding the regulation of this gene. In addition, having only an XbaI site in the resulting fused part ([https://parts.igem.org/Part:BBa_K1983015 K1983015]), it can be still be combined with other biobricks when digested with SpeI and PstI.
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Since one of the fused parts is a composite biobrick ([https://parts.igem.org/Part:BBa_K1983014 K1983014]) comprised of a promoter, RBS, coding gene and terminator, with XbaI it is more adaptable regarding the regulation of this gene. In addition, having only an XbaI site in the resulting fused part ([https://parts.igem.org/Part:BBa_K1983015 K1983015]), it can be still be combined with other biobricks when digested with SpeI and PstI.
  
 
===Primers===
 
===Primers===
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===Source===
 
===Source===
  
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BBa_K1983015
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This part is derived from ([https://parts.igem.org/Part:BBa_B0017 BBa_B0017]). Other parts are derived Escherichia coli and were synthesized by Integrate DNA Technologies.
  
 
===References===
 
===References===

Latest revision as of 21:36, 22 October 2016


PheP and tRNA-Phe under constitutive promoters


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 98
    Illegal NheI site found at 1669
    Illegal NheI site found at 1692
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has an XbaI site between RBS (J61132) and PheP gene (K1983013) because it was created by fusing (K1983014) with (K1983011).

Since one of the fused parts is a composite biobrick (K1983014) comprised of a promoter, RBS, coding gene and terminator, with XbaI it is more adaptable regarding the regulation of this gene. In addition, having only an XbaI site in the resulting fused part (K1983015), it can be still be combined with other biobricks when digested with SpeI and PstI.

Primers

Primers used for amplification of the fragment:
Uni-FW: GTAGAATTCGCGGCCGCTTCTAG
Uni-RV: GTAGACTGCAGCGGCCGCTACTAG

Primers used for colony PCR screening:

For pSB1C3:
VF2: tgccacctgacgtctaagaa
VR: attaccgcctttgagtgagc


Source

This part is derived from (BBa_B0017). Other parts are derived Escherichia coli and were synthesized by Integrate DNA Technologies.

References