Difference between revisions of "Part:BBa K1943014:Design"
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Latest revision as of 03:29, 22 October 2016
Bleo
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 270
Illegal NgoMIV site found at 331 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This year we use mammalian cells as carriers. So in our plasmids, there are several kinds of anti-antibiotics genes which are used for screening. We design primers and do PCR of this anti-antibiotics genes coding sequence and ligate it to pSB1C3 backbone.
Source
The plasmid was from our instructor, Huangwei's lab. And we design primers to copy down by PCR.