Difference between revisions of "Part:BBa K1983000:Design"
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Primers used for amplification of the fragment: <br> | Primers used for amplification of the fragment: <br> | ||
+ | |||
PAL-Chis-ex-FW: AACGTCTCCCATGAAGACCCTGTCCCAG <br> | PAL-Chis-ex-FW: AACGTCTCCCATGAAGACCCTGTCCCAG <br> | ||
PAL-Chis-ex-RV: AACTCGAGATGCAGGCTAGGCAGG <br> | PAL-Chis-ex-RV: AACTCGAGATGCAGGCTAGGCAGG <br> | ||
Line 23: | Line 24: | ||
Up-A1: GGATCTCGACGCTCTCCCT <br> | Up-A1: GGATCTCGACGCTCTCCCT <br> | ||
Down3: ACCCCTCAAGACCCGTTTAG <br> | Down3: ACCCCTCAAGACCCGTTTAG <br> | ||
+ | |||
+ | Primers used for cloning into biobrick: <br> | ||
+ | |||
+ | PAL-Chis-BBa-FW: AATCTAGatgaagaccctgtcccagg <br> | ||
+ | PAL-Chis-BBa-RV: AAACTAGTATTAGTGATGGTGGTGGTGATG <br> | ||
===Source=== | ===Source=== | ||
− | + | This part is an improvement of ([https://parts.igem.org/Part:BBa_K1692004 BBa_K1692004]) and was synthesized by Integrate DNA Technologies | |
===References=== | ===References=== |
Latest revision as of 10:37, 22 October 2016
Codon optimized AvPAL with C-terminal 6XHis-Tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1265
Illegal XhoI site found at 1702 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1483
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primers
Primers used for amplification of the fragment:
PAL-Chis-ex-FW: AACGTCTCCCATGAAGACCCTGTCCCAG
PAL-Chis-ex-RV: AACTCGAGATGCAGGCTAGGCAGG
Primers used for colony PCR screening:
For pBAD:
Bad-P1: AGATTAGCGGATCCTACCTGACGC
Bad-T1: TGTTTTATCAGACCGCTTCTGCGTTC
For pETDuet-1:
Up-A1: GGATCTCGACGCTCTCCCT
Down3: ACCCCTCAAGACCCGTTTAG
Primers used for cloning into biobrick:
PAL-Chis-BBa-FW: AATCTAGatgaagaccctgtcccagg
PAL-Chis-BBa-RV: AAACTAGTATTAGTGATGGTGGTGGTGATG
Source
This part is an improvement of (BBa_K1692004) and was synthesized by Integrate DNA Technologies