Difference between revisions of "Part:BBa K1992010"

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==Design considerations==
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==Experiments and results==
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A flexible linker was introduced to fuse the GFP gene to the chimera. The linker is essential for the protein to fold in the right manner and for the correct tertiary structure to be obtained.
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For methods and results please go to PctA-Tar (<partinfo>BBa_K1992001</partinfo>) or to our [http://2016.igem.org/Team:Technion_Israel/Proof proof of concept] page
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For methods and results please go to PctA-Tar (<partinfo>BBa_K1992001</partinfo>)
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Latest revision as of 13:52, 21 October 2016

PctA-Tar GFP tagged expression system (promoter+RBS+coding+terminator)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 331
    Illegal NgoMIV site found at 412
    Illegal NgoMIV site found at 416
    Illegal NgoMIV site found at 435
    Illegal AgeI site found at 535
    Illegal AgeI site found at 746
    Illegal AgeI site found at 1601
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2657


Introduction

A GFP gene was fused to the PctA chimera. The plasmid is comprised of a promoter, a strong RBS, the chimera, a GFP gene and a terminator. In addition, a proper linker ought to be introduced in order to ensure the right correct fold of the protein.


Usage and Biology

This expression device was used as a proof of concept in order to verify the migration of the new chemoreceptor to the poles of the bacterial membrane.


Experiments and results

For methods and results please go to PctA-Tar (BBa_K1992001) or to our [http://2016.igem.org/Team:Technion_Israel/Proof proof of concept] page