Difference between revisions of "Part:BBa K416001"
 
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<h1>'''Improvement by 2016 NCTU_Formosa'''</h1>
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: (links to uploads relevant to your contribution,
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<h1>'''Contribution'''</h1>
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<b><p>Group: NCTU_Formosa 2016 </p></b>
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<b><p>Author: YU-CHUN WU</p></b>
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<b>
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Summary:</b>
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<br>
 
<p>In the experiment, we hypothesized that an elongated linker would enhance the function of the fusion protein for the original linker is too short for the proper folding of the two domains. Furthermore, we found no result in the previous history using the Flexible Peptide GS Linker. Therefore, we modified the sequence and added three more amino acids from into the linker. In the result, our improved GS Linker is completely proved by our experiments.</p>   
 
<p>In the experiment, we hypothesized that an elongated linker would enhance the function of the fusion protein for the original linker is too short for the proper folding of the two domains. Furthermore, we found no result in the previous history using the Flexible Peptide GS Linker. Therefore, we modified the sequence and added three more amino acids from into the linker. In the result, our improved GS Linker is completely proved by our experiments.</p>   
  
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<p style="padding-top:20px;">
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<h3><b>New improved part
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<b>Result and Experiment</b></p>
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<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1974030">BBa_K1974030</a></html></b></h3>
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<p style=“padding:1px;”>  
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<br>
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In the experiment, the fusion protein with the elongated GS linker did show the enhanced repellent effect. Observed from the phenomenon, we did prove the enhanced function of the fusion proteins(see Result), so we speculate that the fusion protein has a better secondary structure than the original ones. As a result, we have submitted a basic part for the improved elongated GS linker.
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<b>Result and Experiment</b>
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[[File:GS_linker_PCR_resullts.png|200px|thumb|center|'''Figure 2.'''GS linker.
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<br>
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In the experiment, the fusion protein with the elongated GS linker did show the enhanced repellent effect. Observed from the phenomenon, we did prove the enhanced function of the fusion proteins, so we speculate that the fusion protein has a better secondary structure than the original ones. As a result, we have submitted a basic part for the improved elongated GS linker.</p>
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[[File:GS_linker_PCR_resullts.png|200px|thumb|center|'''Figure. '''GS linker.
 
The DNA sequence length of GS linker is around 50-60 b.p. In this PCR experiment, the product’s size should be close to 300-350 b.p.]]
 
The DNA sequence length of GS linker is around 50-60 b.p. In this PCR experiment, the product’s size should be close to 300-350 b.p.]]
  
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<p style="padding-top:20px;">
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<br>
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<b>Application of this part</b></p>
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<b>Uploads</b>
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<br>
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<b>For more result information on how the function of our GS linker worked in our project, see </b>
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<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1974021">BBa_K1974021</a></html>
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<b><html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1974033">BBa_K1974033</a></html></b>
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<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1974021">BBa_K1974021</a></html>
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<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1974023">BBa_K1974023</a></html>
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Latest revision as of 09:45, 21 October 2016


(Gly4Ser)3 Flexible Peptide Linker

This is a 15 amino acid flexible peptide linker protein domain that is useful for creating functional fusion proteins. The linker is to be fused in frame in between two protein domains, separating the two domains so that they each retain original functions yet will be physically connected.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


(links to uploads relevant to your contribution,

Contribution

Group: NCTU_Formosa 2016

Author: YU-CHUN WU

Summary:

In the experiment, we hypothesized that an elongated linker would enhance the function of the fusion protein for the original linker is too short for the proper folding of the two domains. Furthermore, we found no result in the previous history using the Flexible Peptide GS Linker. Therefore, we modified the sequence and added three more amino acids from into the linker. In the result, our improved GS Linker is completely proved by our experiments.

New improved part BBa_K1974030


Result and Experiment
In the experiment, the fusion protein with the elongated GS linker did show the enhanced repellent effect. Observed from the phenomenon, we did prove the enhanced function of the fusion proteins, so we speculate that the fusion protein has a better secondary structure than the original ones. As a result, we have submitted a basic part for the improved elongated GS linker.</p>

Figure. GS linker. The DNA sequence length of GS linker is around 50-60 b.p. In this PCR experiment, the product’s size should be close to 300-350 b.p.


Uploads
For more result information on how the function of our GS linker worked in our project, see BBa_K1974033