Difference between revisions of "Part:BBa K1955006"
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| − | <b style="font-size:23px;"> | + | <b style="font-size:23px;">pSB1C3-5'HYG-OVA-3'UTR </b><br><br> |
OVA has been commonly used as the antigen for testing the efficiency of antibody response and T cell activation in previous immunology experiments, thus is a easy to use antigen. We add the OVA coding region in between the Leish-5'UTR-HYG and Leish-3'UTR for leishmania to expression our target protein, OVA. | OVA has been commonly used as the antigen for testing the efficiency of antibody response and T cell activation in previous immunology experiments, thus is a easy to use antigen. We add the OVA coding region in between the Leish-5'UTR-HYG and Leish-3'UTR for leishmania to expression our target protein, OVA. | ||
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<img src="https://static.igem.org/mediawiki/2016/2/2d/CGU_Taiwan--bio6.jpg" width=550px height=600px style="border:2px black solid;border-radius:8px;"></img><br><br> | <img src="https://static.igem.org/mediawiki/2016/2/2d/CGU_Taiwan--bio6.jpg" width=550px height=600px style="border:2px black solid;border-radius:8px;"></img><br><br> | ||
Latest revision as of 05:42, 21 October 2016
pSB1C3-5'HYG-OVA-3'UTR
OVA has been commonly used as the antigen for testing the efficiency of antibody response and T cell activation in previous immunology experiments, thus is a easy to use antigen. We add the OVA coding region in between the Leish-5'UTR-HYG and Leish-3'UTR for leishmania to expression our target protein, OVA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1458
Illegal BamHI site found at 3562 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 2689