Difference between revisions of "Part:BBa K1900000:Design"
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===Source=== | ===Source=== | ||
| − | + | BBa_K1406000 comes from the BioBrick registry The tolC signaling sequence and Klebsiella pneumoniae tolC sequence were synthesized from DNA sequences found in genomic databases. | |
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===References=== | ===References=== | ||
Latest revision as of 16:52, 20 October 2016
pBAD+strong RBS+E. coli tolC signal sequence+K. pneumoniae tolC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1603
Illegal NheI site found at 1624 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1543
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1104
Design Notes
BBa_K206000 was chosen for its ability to be controlled with a common inducer (arabinose) as well as its high rate of transcription. BBa_B0034 was chosen for its high translation efficiency. The main consideration when designing the signaling sequence and Klebsiella pneumoniae TolC gene was to use silent mutations alter any RFC10 incompatible DNA sections to be BioBrick compatible.
Source
BBa_K1406000 comes from the BioBrick registry The tolC signaling sequence and Klebsiella pneumoniae tolC sequence were synthesized from DNA sequences found in genomic databases.