Difference between revisions of "Part:BBa K2082003"
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<partinfo>BBa_K2082003 short</partinfo> | <partinfo>BBa_K2082003 short</partinfo> | ||
| − | Anderson promoter, RBS, Fluorescent protein (GFP) CDS and Split fluorescent protein CDS | + | Anderson promoter, RBS, Fluorescent protein (GFP) CDS and Split fluorescent protein (CFP) CDS |
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<li>a C-terminal split-CFP CDS</li> | <li>a C-terminal split-CFP CDS</li> | ||
</ul> | </ul> | ||
| + | <br> | ||
| + | This device can be used in combination with <a href="https://parts.igem.org/Part:BBa_K2082002">BBa_K2082002</a> to evaluate the binding properties of the anti-GFP-Nanobody. The attachment of GFP and the anti-GFP-Nanobody leads to the completion of the split CFP domains. and so to a blue fluorescence. | ||
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| + | <figure class="figure"> | ||
| + | <img src="https://static.igem.org/mediawiki/parts/e/ed/Bielefeld_CeBiTec_2016_10_20_bifc_gfp.png" class="figure-img" alt="" width=60%> | ||
| + | <figcaption class="figure-caption"><b><i>E. coli</i> with BBa_K2082003. Green fluorescence, caused by GFP.</b></figcaption> | ||
| + | </figure> | ||
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</html> | </html> | ||
Latest revision as of 17:35, 20 October 2016
Split CFP Bimolecular fluorescence complementation (BiFC) with GFP
Anderson promoter, RBS, Fluorescent protein (GFP) CDS and Split fluorescent protein (CFP) CDS
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 86
Illegal AgeI site found at 209 - 1000COMPATIBLE WITH RFC[1000]
This device consists of:
- an Anderson promoter (BBa_J23106)
- a RBS (BBa_B0034)
- a GFP CDS
- a C-terminal split-CFP CDS
This device can be used in combination with BBa_K2082002 to evaluate the binding properties of the anti-GFP-Nanobody. The attachment of GFP and the anti-GFP-Nanobody leads to the completion of the split CFP domains. and so to a blue fluorescence.
