Difference between revisions of "Part:BBa K2070005"
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<partinfo>BBa_K2070005 short</partinfo> | <partinfo>BBa_K2070005 short</partinfo> | ||
− | This part was used to test the functionality of ECF sigma35 and its cognate promoter. | + | This part, consisting of a constitutive ECF sigma35 generator [https://parts.igem.org/Part:BBa_K2070013 BBa_K2070013] and ECF sigma35 inducible GFP reporter [https://parts.igem.org/Part:BBa_K2070004 BBa_K2070004], was used to test the functionality of ECF sigma35 and its cognate promoter. See experience page for details of the result. |
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+ | [[File:T--UT-Tokyo--BBa K2070005--3.png|500px]] | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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− | + | [[File:T--UT-Tokyo--BBa K2070005-1.png|thumb|centre|700px|'''Measurement of ECF sigma35 and sigma30 promoter activity. '''Fluorescence/OD600 of a construct containing both an ECF sigma35 generating device <partinfo>BBa_K2070013</partinfo> and ECF sigma 35 inducible GFP generating device <partinfo>BBa_K2070005</partinfo> was compared to that containing only either one of the devices. *** for P> 0.001 indicates an extremely significant difference between the induced and uninduced state. ]] | |
− | <partinfo>BBa_K2070005 | + | |
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+ | <!-- Add more about the biology of this part here | ||
+ | ===Usage and Biology=== | ||
Latest revision as of 07:18, 25 October 2016
Constitutive ecf35 generator with ecf35 inducible GFP reporter
This part, consisting of a constitutive ECF sigma35 generator BBa_K2070013 and ECF sigma35 inducible GFP reporter BBa_K2070004, was used to test the functionality of ECF sigma35 and its cognate promoter. See experience page for details of the result.
Usage and Biology
![](/wiki/images/c/cc/T--UT-Tokyo--BBa_K2070005-1.png)
Measurement of ECF sigma35 and sigma30 promoter activity. Fluorescence/OD600 of a construct containing both an ECF sigma35 generating device BBa_K2070013 and ECF sigma 35 inducible GFP generating device BBa_K2070005 was compared to that containing only either one of the devices. *** for P> 0.001 indicates an extremely significant difference between the induced and uninduced state.