Difference between revisions of "Part:BBa K1906003"
 
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<partinfo>BBa_K1906003 short</partinfo>
 
<partinfo>BBa_K1906003 short</partinfo>
  
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LtrA is a protein that encoded by <i>Lactococcus lactis</i> LtrB intron. LtrA is a multifunctional protein that promotes group II intron splicing and mobility.
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LtrA is a intron-encoded protein (IEP) from <i>Lactococcus lactis</i>. LtrA is a multifunctional protein that promotes group II intron splicing and mobility.
  
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===Usage and Biology===
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This enzyme is a maturase that catalyzes the formation of mature form of introns, a endonuclease once bind with mature RNAs and a reverse transcriptase that can drive the formation of cDNA strand of RNA it carries.
  
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It has three activities: reverse transcriptase (RT) for intron duplication, maturase to promote splicing, and DNA endonuclease for site-specific cleavage of recipient alleles. The intron-encoded protein promotes splicing by facilitating the formation of the catalytically active structure of the intron RNA. After splicing, the protein remains bound to the excised intron lariat RNA, forming ribonucleoprotein particles, and cleaving the antisense strand of the recipient DNA in the 3' exon. After DNA cleavage, retrohoming occurs by a target DNA-primed reverse transcription of the intron RNA that had reverse spliced into the sense strand of the recipient DNA. It also contributes to the recognition of the DNA target site and acts as a repressor of its own translation.
 
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===Usage and Biology===
 
  
 
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Latest revision as of 10:47, 20 October 2016


Intron encoded protein LtrA

LtrA is a intron-encoded protein (IEP) from Lactococcus lactis. LtrA is a multifunctional protein that promotes group II intron splicing and mobility.

Usage and Biology

This enzyme is a maturase that catalyzes the formation of mature form of introns, a endonuclease once bind with mature RNAs and a reverse transcriptase that can drive the formation of cDNA strand of RNA it carries.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1093
    Illegal BamHI site found at 1469
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 369
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 291