Difference between revisions of "Part:BBa K2120424"

Latest revision as of 08:54, 23 October 2016

B0015+cI+B0034+J23109+pR+B0032+mRFP+B0015

This is one of our application circuit.

<Description>
This device is similar to our project final gene circuit using relationship of inhibitor-promoter to start downstream gene’s transcription.We use RFP as a report gene. The report gene could indicate a safety condition when killer gene express.
In order to adjust the repressing efficiency, we choose weak constitutive promoter J23109 and strong RBS B0034 to express inhibitor gene cI. Meanwhile, we also constructed more circuits which have different combine with promoter, RBS and inhibitor. More detail see in our Wiki([http://2016.igem.org/Team:BIT-China/Parts Parts Submission])

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 941
Illegal NheI site found at 964
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1614
Illegal AgeI site found at 1726
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 This is one of our application circuit.
-Our application circuit circuit consists of two parts:the inhibitor device and killer device ,which have already been made into basic parts of biobricks.
-We choose four different promoters whose reported strengths are respectively low, medium,strong and the strongest from constitutive promoter family to express the inhibitor tetR and cI ,and then we construct three plasmid with different copy number containing this circuit, in order to find out the proper promoter which meets the threshold that trigger the killer system.
+'''<Description>'''
+<br>This device is similar to our project final gene circuit using relationship of inhibitor-promoter to start downstream gene’s transcription.We use ''RFP'' as a report gene. The report gene could indicate a safety condition when killer gene express.
-Go our [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2016&group=BIT-China Parts Registry] to learn more.
+<br>In order to adjust the repressing efficiency, we choose weak constitutive promoter J23109 and strong RBS B0034 to express inhibitor gene ''cI''. Meanwhile, we also constructed more circuits which have different combine with promoter, RBS and inhibitor. More detail see in our Wiki([http://2016.igem.org/Team:BIT-China/Parts Parts Submission])