Difference between revisions of "Part:BBa K2120410"
 
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This is one of our application circuit.
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'''<Description>'''
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<br>This is one of our application circuits.
  
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Our application circuit circuit consists of two parts:the inhibitor device and killer device ,which have already been made into basic parts of biobricks.
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<br>This circuit contains two devices: inhibitor device and killer device.  
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<br>Inhibitor device: We select medium weak constitutive promoter J23116 and strong RBS B0034 to express inhibitor gene ''tetR''.
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We choose four different promoters whose reported strengths are respectively low, medium,strong and the strongest from constitutive promoter family to express the inhibitor tetR and cI ,and then we construct three plasmid with different copy number containing this circuit, in order to find out the proper promoter which meets the threshold that trigger the killer system.
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<br>Killer device: Toxin protein, MazF, can cleavage ACA sequence of free mRNA specifically. It cause bacteria can’t translate successfully and dead finally. So we constructed it with induced promoter pTet and medium RBS B0032 to control the expression level of toxin protein.
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<br>We reversed the direction of inhibitor device, in order to reduce the influence between two devices.
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Go our [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2016&group=BIT-China Parts Registry] to learn more.
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<br>Meanwhile, we also constructed more circuits which have different combine with promoter, RBS and inhibitor. More detail see in our Wiki([http://2016.igem.org/Team:BIT-China/Parts Parts Submission])
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 12:40, 23 October 2016


B0015+tetR+B0034+J23116+ptet+B0032+mazF+B0015


<Description>
This is one of our application circuits.


This circuit contains two devices: inhibitor device and killer device.
Inhibitor device: We select medium weak constitutive promoter J23116 and strong RBS B0034 to express inhibitor gene tetR.
Killer device: Toxin protein, MazF, can cleavage ACA sequence of free mRNA specifically. It cause bacteria can’t translate successfully and dead finally. So we constructed it with induced promoter pTet and medium RBS B0032 to control the expression level of toxin protein.
We reversed the direction of inhibitor device, in order to reduce the influence between two devices.
Meanwhile, we also constructed more circuits which have different combine with promoter, RBS and inhibitor. More detail see in our Wiki([http://2016.igem.org/Team:BIT-China/Parts Parts Submission])


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 851
    Illegal NheI site found at 874
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]