Difference between revisions of "Part:BBa K2150115"
 
(3 intermediate revisions by the same user not shown)
Line 6: Line 6:
 
We characterized this by measuring the growth curve co-expressing toxin and antitoxin.
 
We characterized this by measuring the growth curve co-expressing toxin and antitoxin.
  
−
[[file:T--UCAS--6249.png|500px]]
+
This protein is induced by aTc. When studying the interaction between antitoxin and toxin, antitoxin is induced by IPTG.
 +
 
 +
See: [https://parts.igem.org/Part:BBa_K2150117 BBa_K2150117]
 +
 
 +
We also characterized its corresponding toxin, ParE, see:
 +
[https://parts.igem.org/Part:BBa_K2150111 BBa_K2150111]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 02:55, 20 October 2016


6249 antitoxin tet

This antitoxin belongs to ParD family. It can effectively neutralize the toxin ParE. We characterized this by measuring the growth curve co-expressing toxin and antitoxin.

This protein is induced by aTc. When studying the interaction between antitoxin and toxin, antitoxin is induced by IPTG.

See: BBa_K2150117

We also characterized its corresponding toxin, ParE, see: BBa_K2150111

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 357
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]