Difference between revisions of "Part:BBa K2066053:Design"
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The part was made to put K2066022 and K2066023 on the same plasmid. | The part was made to put K2066022 and K2066023 on the same plasmid. | ||
Latest revision as of 02:28, 20 October 2016
pTET GFP + TetR on UNS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1004
Illegal NheI site found at 1027 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 764
Design Notes
The part was made to put K2066022 and K2066023 on the same plasmid.
Source
This part was derived mostly from iGEM registry parts. It is a combination of K2066022 and K2066023. It uses UNS sequences from Torella et. al 2013 ("Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly")