Difference between revisions of "Part:BBa K1886018"
 
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<h2>'''Characterization'''</h2>
 
<h2>'''Characterization'''</h2>
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<h3> BACKGROUND </h3>
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<h4>Principle</h4>
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<h4>Overview</h4>
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Considering that PompC is a red light inhibited promoter, we designed a NOT Gate to realize our purpose of a light-activating system.
 
Considering that PompC is a red light inhibited promoter, we designed a NOT Gate to realize our purpose of a light-activating system.
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<h4>Principle</h4>
 
 
Pλ is a promoter which can be inhibited by protein CI. We use this part combined with CI to realize the main function of regulating the whole system.
 
Pλ is a promoter which can be inhibited by protein CI. We use this part combined with CI to realize the main function of regulating the whole system.
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<h3>RESULTS</h3>
 
 
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<h4>Gel electrophoretic analysis</h4>
 
<h4>Gel electrophoretic analysis</h4>
 
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[[File:Pompc_CI_p-lamda_sfGFP.jpg|800px|thumb|left|Fig.1 Fig.1 Gel electrophoretic analyses of PCR products ]]
  
 
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Latest revision as of 00:32, 20 October 2016


Pompc_CI_p-lamda_sfGFP

We inserted a CI protein-terminator-Pλ between Pompc and sfGFP to change the red-TCS from being activated by darkness and inhibited by red light into being activated by red light but inhibited by darkness.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1141


Characterization

Principle

Considering that PompC is a red light inhibited promoter, we designed a NOT Gate to realize our purpose of a light-activating system. Pλ is a promoter which can be inhibited by protein CI. We use this part combined with CI to realize the main function of regulating the whole system.

Gel electrophoretic analysis


Fig.1 Fig.1 Gel electrophoretic analyses of PCR products