Difference between revisions of "Part:BBa K2150017"
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<partinfo>BBa_K2150017 short</partinfo> | <partinfo>BBa_K2150017 short</partinfo> | ||
| − | T7 promoter with RBS, and the fusion protein of tetX and GFP(referred to as tetX-GFP BBa_K2150013) with double terminator. | + | T7 promoter with RBS, and the fusion protein of tetX and GFP(referred to as tetX-GFP, BBa_K2150013) with double terminator. |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This part is designed to produce a high level of the degrading enzyme in the presence of T7 RNA polymerase(see [https://parts.igem.org/Part:BBa_K2150028 BBa_K21500028]). | + | This part is designed to produce a high level of the tetracycline-degrading enzyme in the presence of T7 RNA polymerase(see [https://parts.igem.org/Part:BBa_K2150028 BBa_K21500028]). |
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Latest revision as of 01:22, 20 October 2016
pT7 TetX-GFP(fusion protein)
T7 promoter with RBS, and the fusion protein of tetX and GFP(referred to as tetX-GFP, BBa_K2150013) with double terminator.
Usage and Biology
This part is designed to produce a high level of the tetracycline-degrading enzyme in the presence of T7 RNA polymerase(see BBa_K21500028).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 608
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1905