Difference between revisions of "Part:BBa I20248"

Latest revision as of 13:30, 16 August 2007

Device used to characterize Ribosome Binding Sites through measurement of GFP expression

Device is based on I2055 and contains a partial biobrick site (EcoR1, Not1, Xba1) between R0040 and GFP. To insert a BB promoter for testing, digest the promoter EcoR1 and Spe1 and the kit EcoR1 and Xba1. The result will form a full BB prefix upstream of the RBS and a mixed site downstream. The full BB prefix has a larger spacing than would occur in actual construction.

Note: sequence features are wrong

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 61
Illegal XbaI site found at 75
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 61
Illegal NotI site found at 67
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 61
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 61
Illegal XbaI site found at 75
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 61
Illegal XbaI site found at 75
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 723

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 __NOTOC__
 <partinfo>BBa_I20248 short</partinfo>
 Device is based on I2055 and contains a partial biobrick site (EcoR1, Not1, Xba1) between R0040 and GFP. To insert a BB promoter for testing, digest the promoter EcoR1 and Spe1 and the kit EcoR1 and Xba1. The result will form a full BB prefix upstream of the RBS and a mixed site downstream. The full BB prefix has a larger spacing than would occur in actual construction.
+Note: sequence features are wrong
 <!-- Add more about the biology of this part here