Difference between revisions of "Part:BBa K2036008"
 
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<partinfo>BBa_K2036008 short</partinfo>
 
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RBS-CIII-RBS-CIII,as Ftsh normally exists in E.coli to degrade CII, CIII functions as a inhibitor of Ftsh, and tandom expression of CIII,may enhance the effect on Ftsh  
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As Ftsh normally exists in E.coli to degrade CII, CIII functions as an inhibitor of Ftsh, and tandem expression of CIII can repress the effect of Ftsh.
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<h2>Protein&protein reaction</h2>
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We had submitted and documented RBS-CIII-RBS-CIII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag ([https://parts.igem.org/Part:BBa_K2036014 BBa_K2036014])  and RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag ([https://parts.igem.org/Part:BBa_K2036015 BBa_K2036015]). These two parts were constructed to test whether CIII can protect CII from being degraded by Ftsh by competitive inhibition.
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[[File:T--HUST-China--CIII%26Ftsh.png|800px|thumb|center|Fig1: According to the Fluorescence measurement curve above, we can see clearly that GFP level of the CIII test circuit increases over time and it shows significant difference from two control groups. It indicates that tandemly expressed CIII can efficiently protect CII from being degraded by Ftsh. ]]
  
 
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<partinfo>BBa_K2036008 parameters</partinfo>
 
<partinfo>BBa_K2036008 parameters</partinfo>
 
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<h2>Protein&protein reaction</h2>
 
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<p>
 
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We had submitted and documented RBS-CIII-RBS-CIII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag (BBa_K2036014) and RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag (BBa_K2036015). These two parts were to test whether CIII can protect CII from being degraded by Ftsh by competitive inhibition.
 
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</p>
 
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[[File:T--HUST-China--CIII%26Ftsh.png|800px|thumb|center|Fig1: According to the Flourescence measurement curve above, we can see clearly that GFP level of CIII test circuit increased over time and it showed significant difference from two control groups. It indicates that tandomly expressed CIII can efficiently protect CII from being degraded by Ftsh. ]]
 

Latest revision as of 05:24, 25 October 2016


RBS-CIII-RBS-CIII,tandem expression

As Ftsh normally exists in E.coli to degrade CII, CIII functions as an inhibitor of Ftsh, and tandem expression of CIII can repress the effect of Ftsh.

Protein&protein reaction

We had submitted and documented RBS-CIII-RBS-CIII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag (BBa_K2036014) and RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag (BBa_K2036015). These two parts were constructed to test whether CIII can protect CII from being degraded by Ftsh by competitive inhibition.


Fig1: According to the Fluorescence measurement curve above, we can see clearly that GFP level of the CIII test circuit increases over time and it shows significant difference from two control groups. It indicates that tandemly expressed CIII can efficiently protect CII from being degraded by Ftsh.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]