Difference between revisions of "Part:BBa K2170050:Design"
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'''Related BioBrick:''' | '''Related BioBrick:''' | ||
| − | * | + | *Related BioBricks: |
| − | + | [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170052 BBa_K2170052: Secretory prokaryotic biotin binding receptor with single chain avidin]<br> | |
| − | + | [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170050 BBa_K2170050: Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin]<br> | |
'''Cloning details:'''<br> | '''Cloning details:'''<br> | ||
*Designed in RFC10 | *Designed in RFC10 | ||
| Line 25: | Line 25: | ||
'''Quality control measures:'''<br> | '''Quality control measures:'''<br> | ||
| − | + | *Test digestion using EcoRI & PstI | |
| − | + | *Sequencing using primer VF2 & VR | |
| − | + | *Part was partly sequenced/Part was totally sequenced | |
'''Backbone:'''<br> | '''Backbone:'''<br> | ||
| Line 35: | Line 35: | ||
'''Protein coding:'''<br> | '''Protein coding:'''<br> | ||
| − | *Protein: Secretory prokaryotic biotin | + | *Protein: Secretory prokaryotic biotin presenting receptor with biotin acceptor peptide [Nucleotide 1030 to 2652]--> |
*Tag: internal A3C5 Tag | *Tag: internal A3C5 Tag | ||
'''Enzymatic activity:''' | '''Enzymatic activity:''' | ||
| − | * | + | * NanoLuc |
'''Cytotoxicity:'''<br> | '''Cytotoxicity:'''<br> | ||
| Line 47: | Line 47: | ||
Known and anticipated sefety issues: none | Known and anticipated sefety issues: none | ||
Known and anticipated security issues: none | Known and anticipated security issues: none | ||
| − | |||
| − | |||
===Source=== | ===Source=== | ||
| Line 54: | Line 52: | ||
'''Source:'''<br> | '''Source:'''<br> | ||
| − | *Preexisting BioBrick BBa_I739001; BBa_B0015 | + | *Preexisting BioBrick BBa_I739001; BBa_B0015; BBa_K1159001 |
*Parts Synthesized by IDT | *Parts Synthesized by IDT | ||
*Professor Dr. Arne Skerra (Chair biological chemistry of TUM) | *Professor Dr. Arne Skerra (Chair biological chemistry of TUM) | ||
| Line 73: | Line 71: | ||
'''Literature references:'''<br> | '''Literature references:'''<br> | ||
| − | + | ||
| + | 1. [http://jcb.rupress.org/content/108/2/229.short Kozak, M. (1989). The scanning model for translation: an update. The Journal of cell biology, 108(2), 229-241.]<br> | ||
| + | 2. [http://journals.plos.org/plosone/article?id=10.1371/journal.pone.000439 Kredel, S., Oswald, F., Nienhaus, K., Deuschle, K., Röcker, C., Wolff, M., ... & Wiedenmann, J. (2009). mRuby, a bright monomeric red fluorescent protein for labeling of subcellular structures. PloS one, 4(2), e4391.]<br> | ||
| + | 3. [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4754705/ Bajar, B. T., Wang, E. S., Lam, A. J., Kim, B. B., Jacobs, C. L., Howe, E. S., ... & Chu, J. (2016). Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting. Scientific reports, 6.]<br> | ||
| + | 4. [http://www.nature.com/nprot/journal/v2/n6/abs/nprot.2007.209.html Schmidt, T. G., & Skerra, A. (2007). The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins. Nature protocols, 2(6), 1528-1535.]<br> | ||
'''Database references:'''<br> | '''Database references:'''<br> | ||
Latest revision as of 16:31, 19 October 2016
Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2448
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 889
Keywords:
Abbreviations:
Design Notes
Related BioBrick:
- Related BioBricks:
BBa_K2170052: Secretory prokaryotic biotin binding receptor with single chain avidin
BBa_K2170050: Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin
Cloning details:
- Designed in RFC10
Quality control measures:
- Test digestion using EcoRI & PstI
- Sequencing using primer VF2 & VR
- Part was partly sequenced/Part was totally sequenced
Backbone:
- Backbone name: pSB1C3
- Resistance: Cp
- Copynumber: high
Protein coding:
- Protein: Secretory prokaryotic biotin presenting receptor with biotin acceptor peptide [Nucleotide 1030 to 2652]-->
- Tag: internal A3C5 Tag
Enzymatic activity:
- NanoLuc
Cytotoxicity:
- none
Safety notes:
Known and anticipated sefety issues: none
Known and anticipated security issues: none
Source
Source:
- Preexisting BioBrick BBa_I739001; BBa_B0015; BBa_K1159001
- Parts Synthesized by IDT
- Professor Dr. Arne Skerra (Chair biological chemistry of TUM)
Organism:
Genesequence derived from Escherichia coli
- Designed for the following Chassis: procaryotic cells
References
Literature references:
1. [http://jcb.rupress.org/content/108/2/229.short Kozak, M. (1989). The scanning model for translation: an update. The Journal of cell biology, 108(2), 229-241.]
2. [http://journals.plos.org/plosone/article?id=10.1371/journal.pone.000439 Kredel, S., Oswald, F., Nienhaus, K., Deuschle, K., Röcker, C., Wolff, M., ... & Wiedenmann, J. (2009). mRuby, a bright monomeric red fluorescent protein for labeling of subcellular structures. PloS one, 4(2), e4391.]
3. Bajar, B. T., Wang, E. S., Lam, A. J., Kim, B. B., Jacobs, C. L., Howe, E. S., ... & Chu, J. (2016). Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting. Scientific reports, 6.
4. [http://www.nature.com/nprot/journal/v2/n6/abs/nprot.2007.209.html Schmidt, T. G., & Skerra, A. (2007). The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins. Nature protocols, 2(6), 1528-1535.]
Database references: