Difference between revisions of "Part:BBa K1976004:Design"

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===Design Notes===
 
===Design Notes===
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As a source for the attP-sequence BBa_I11023 was used. After further research we realized that this BioBrick is not carrying the exact attachment site of λ-phage. Instead, the sequence corresponds to the attachment site used in the Gateway &erg; cloning system. We had to change three nucleotides by mutagenesis PCR to receive the accurate &lambda-phage attP sequence.
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As a source for the <i>attP</i>-sequence BBa_I11023 was used. After further research we realized that this BioBrick is not carrying the exact attachment site of &lambda;-phage. Instead, the sequence corresponds to the attachment site used in the Gateway &reg; cloning system. We had to change three nucleotides by mutagenesis PCR to receive the accurate &lambda;-phage <i>attP</i>-sequence.
  
 
===Source===
 
===Source===

Latest revision as of 13:17, 19 October 2016


λ-attP site flanked with two bidirectional terminators


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As a source for the attP-sequence BBa_I11023 was used. After further research we realized that this BioBrick is not carrying the exact attachment site of λ-phage. Instead, the sequence corresponds to the attachment site used in the Gateway ® cloning system. We had to change three nucleotides by mutagenesis PCR to receive the accurate λ-phage attP-sequence.

Source

Synthesis by iDT

References