Difference between revisions of "Part:BBa K2130005"

 
 
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Human codon optimised NmCas9
 
Human codon optimised NmCas9
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NmCas9 is originated from Neisseria meningitidis species with a PAM requirement of NGGNGATT that placed at the 3' end of spacer sequence. From the results attached, it shows that NmCas9 is generally not performed well for both NHEJ and HDR even with different spacer length that range from 17-23 nucleotides.
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<html><center><img src="https://static.igem.org/mediawiki/parts/0/0b/NmCas9.PNG" height="60%"width="75%">
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<img src="https://static.igem.org/mediawiki/2016/f/fc/T--NTU-Singapore--bigtest.jpg" height="60%"width="75%"></center>
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<img src="https://static.igem.org/mediawiki/2016/a/af/T--NTU-Singapore--hdr.jpg" height="60%"width="75%"></center>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 12:20, 24 October 2016


NmCas9

Human codon optimised NmCas9

NmCas9 is originated from Neisseria meningitidis species with a PAM requirement of NGGNGATT that placed at the 3' end of spacer sequence. From the results attached, it shows that NmCas9 is generally not performed well for both NHEJ and HDR even with different spacer length that range from 17-23 nucleotides.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 511
    Illegal NgoMIV site found at 3282
    Illegal NgoMIV site found at 3301
    Illegal AgeI site found at 3271
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1373
    Illegal BsaI.rc site found at 2557